Journal: International Immunology

Article Title: Antibody-secreting cells in respiratory tract tissues in the absence of eosinophils as supportive partners

doi: 10.1093/intimm/dxw035

Figure Lengend Snippet: ASCs in nasal tissues of mice deficient in eosinophils or neutrophils. (A) Virus-specific ASCs are shown 1 month post-infection for nasal tissues, lung, and CLN of control mice. In the insert panel, a typical FACS profile of anti-CD11b and anti-Siglec antibody-stained blood cells is shown. CD11bHI Siglec+ cells are circled. (B) Virus-specific ASCs are shown 1 month post infection for nasal tissues, lung, and CLN of ∆dblGATA-1 mice. As in A, a typical FACS profile is shown for stained blood cells from ∆dblGATA-1 mice. (C) Virus specific ASCs are shown 4 months post-infection in control animals. (D) Virus-specific ASCS are shown 4 months post-infection in ∆dblGATA-1 mice. (E) Total ASCS are shown in control littermates (for the Mcl1∆M mouse model). A typical FACS profile for control blood stained with anti-CD11b and anti-Gr1 anti bodies is shown in the panel insert. (F) Total ASCs are shown in Mcl1∆M mice. Similar to panel E, a typical FACS profile of stained blood cells from Mcl1∆M mice is shown in the panel insert. Significant and reproducible reductions in values for test mice compared to controls are indicated by asterisks (Student’s t test).

Article Snippet: Sigma–Aldrich Cat#31392-10G) in PBS and cells were stained with anti-CD11b (anti CD11b PerCPCy5.5 1:100 Cat # 550993 BD Pharmingen) and anti-Gr1 (anti-Ly6-G-APC 1:100 Cat# 563129 BD Pharmingen) antibodies for FACS analyses.

Techniques: Infection, Staining

Journal: Cell reports

Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype

doi: 10.1016/j.celrep.2022.111462

Figure Lengend Snippet:

Article Snippet: Anti-Ly-6G/C (Gr-1)-141Pr , Fluidigm , Cat# 3141005B; Clone: RB6-8C5.

Techniques: Control, Recombinant, Liposomes, Labeling, Quantitation Assay, Staining, RNA Sequencing, Mass Spectrometry, Knock-Out, Software